Impact of fluorogenic compounds on biotic production of citric acid

Fluorogenic compounds, i.e., coumarins when used as biotic elicitors or in fermentation media can impact citric acid production by fungi like Aspergillus niger. Research on the impact of coumarins on citric acid fermentation is important for developing new strategies to improve the production of this valuable industrial product. The impact of fluorogenic compound, i.e., 7-hydroxy-4-(trifluoromethyl) coumarin on biotic production of citric acid by Aspergillus fumigatus AR-902, Aspergillus parasiticus AR-904, Aspergillus awamori AR-861, Aspergillus oryzae AR-962 and Aspergillus niger AR-953 has been assessed. It has been found that the fungal strain Aspergillus niger AR-953 has been found most effective for the upgradation of citric acid production. It has been found that the flurogenic compound, i.e.,7-hydroxy-4-(trifluoromethyl) coumarin has stimulatory impact at molar concentration of 4×10–5M and enhances the yield of citric acid to an extent of 6.487% higher in comparison to control, i.e., 6.859 gm/100ml under the optimized parameters viz. 290C temperature, 1.8 pH, 11 days of incubation period and 20% (w/v) molasses solution.