The influence of N-nitrosodiethylamine on novel biotechnological method for production of ethanol by Saccharomyces cerevisiae RK-16

The efficacy of N-nitrosodiethylamine on production of ethanol by Saccharomyces cerevisiae RK-16 has been assessed. It has been found that the mutagen under trial, i,e; N-nitrosodiethylamine at 6.0×10-5 M molar concentration enhances the production of ethanol by Saccharomyces cerevisiae RK-16 to an extent of 20.363% higher in comparison to control fermenter flask, i.e, 5.50 ml/100 in 65 hours of optimum incubation period, 18.5% (w/v) molasses solution 4.5 pH and 300C temperature alongwith other nutritional ingredients required by the yeast.