In vitro microbial production of citric acid by Aspergillus niger NCIM-1270 exposed to 3-acetylcoumarin

The efficacy of 3-acetylcoumarin on in vitro microbial transformation of molasses to citric acid by Aspergillus niger NCIM-1270 has been assessed. It has been found that the coumarin, i.e, 3-acetylcoumarin under trial has stimulatory effect on in vitro microbial transformation of molasses to citric acid by Aspergillus niger NCIM-1270 and enhances the yield of citric acid to an extent of 8.715 % higher in comparison to control fermentor flasks, i.e., 6.955 g/100mL in 12 days of incubation period 2.1 pH and 290C temperature with 24% vol. molasses solution.