Bioethanol production by S. cerevisiae AK-22 exposed to 6-(4-hydroxy-3-methyl-cis-2-butenyl) aminopurine

The influence of cytokinine, i.e., 6-(4-hydroxy-3-methyl-cis-2-butenyl) aminopurine on bioethanol production by the yeast Saccharomyces cerevisiae AK-22 has been assessed. It has been found that the cytokinine, i.e., 6-(4-hydroxy-3-methyl-cis2-butenyl) aminopurine at its molar concentration of 6.0 × 10–5M has stimulatory effect on bioproduction of ethyl alcohol and enhances the yield of bioethanol to an extent of 8.159% higher in comparison to control fermenter flask, i.e. 5.27mL/100 mL while molar concentration of 6-(4-hydroxy-3-methyl-cis-2-butenyl) aminopurine under trial at 6.0 × 10–5 M and onwards inhibits and retards the bioethanol production. The molar concentration of cytokinine, i.e., 6-(4-hydroxy-3-methyl-cis-2-butenyl) aminopurine has been employed in between 1.0 × 10–5M to 10 × 10–5M and has been found that at initial concentration, i.e., 1.0×10–5M it is least effective and at higher concentrations it gives insignificant yield of ethyl alcohol. Experimental parameters has been optimized viz. : 320C temperature, 4.6 pH, 50 hrs incubation period with 16.5% (w/v) molasses solution alongwith other nutritional ingredients required by the yeast Saccharomyces cerevisiae AK-22 .