Bioethanol production by S. cerevisiae AK-22 exposed to 2-ethylphenylhydrazine hydrochloride

The influence of chemical mutagen, i.e., 2-ethylphenylhydrazine hydrochloride on bioethanol production by Saccharomyces cerevisiae AK-22 has been assessed. It has been found that the chemical mutagen, i.e., 2-ethylphenylhydrazine hydrochloride at its molar concentration of 6.0×10–5M has stimulatory effect on bioethanol production and enhances the yield of bioethanol to an extent of 20.113% higher in comparison to control fermenter flask, i.e. 5.27mL/100mL while molar concentration of 2-ethylphenylhydrazine hydrochloride under trial at 7.0×10–5 M and onwards inhibits and retards the bioethanol production. The molar concentration of 2-ethylphenylhydrazine hydrochloride has been employed in between 1.0×10–5M to 10×10–5M and has been found that at initial concentration, i.e., 1.0×10–5M it is least effective and at higher concentrations it gives insignificant yield of ethyl alcohol. Experimental parameters has been optimized viz. : 320C temperature, 4.6 pH, 50hrs incubation period with 16.5% (w/v) molasses solution alongwith other nutritional ingredients required by the yeast Saccharomyces cerevisiae AK-22 .